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The Axiom™ Genotyping Solution

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The Axiom Genotyping Solution is Affymetrix' newest product for genome-wide association studies (GWAS) and candidate gene association studies. It includes array plates with validated genomic content, complete reagent kits, data analysis tools, and a fully automated workflow utilizing the GeneTitan™ Multi-Channel Instrument.

View the ASHG scientific posters for the Axiom Genotyping Solution >

Benefits of the Axiom Genotyping Solution

Flexible content - optimize your studies with a diverse set of relevant and novel variants
Fast results - process more than 750 samples per week on a single platform—the highest throughput in the industry
More power - run more samples within your budget
Greater confidence - generate reliable results with end-to-end automated sample processing and fully optimized reagent kits

The Axiom Genotyping Solution includes:

Axiom™ Genome-Wide Human Array Plates, designed to maximize genomic coverage of common and novel SNP and indels in Caucasian, Asian, and African populations
A proprietary database of validated genomic markers, including HapMap and content from the 1000 Genomes Project, that can be leveraged as part of a custom Axiom design process
Axiom™ Reagent Kits for automated, parallel processing of 96 samples per plate—process between 192 and 768 samples per run
A fully automated workflow with validated robotic methods for target preparation and array processing, based on the Beckman Biomek® FX^P Target Prep Express System and the GeneTitan™ MC Instrument
Genotyping Console™ Software (GTC) with a new Axiom Genotyping Algorithm v1 (Axiom GT1) for automated allele calling and quality assessment of called genotypes

A streamlined assay

Total genomic DNA (200 ng) is amplified and randomly fragmented into 25 to 125 base pair (bp) fragments. These fragments are purified, re-suspended, and hybridized to Axiom Genome-Wide Human Array Plates. Following hybridization, the bound target is washed under stringent conditions to remove non-specific background to minimize background noise caused by random ligation events. Each polymorphic nucleotide is queried via a multi-color ligation event carried out on the array surface. After ligation, the arrays are stained and imaged on the GeneTitan™ Multi-Channel Instrument.

Overview of the Axiom Genome-Wide Genotyping Assay

Overview

For research use only. Not for use in diagnostic procedures.

Brochures

Human genetics with the Axiom® Genotyping Solution (pdf, 41 KB)

Data Sheets

Axiom® Exome Genotyping Array Plates (pdf, 587 KB)

Axiom® Genome-Wide ASI 1 Array Plate (pdf, 867 KB)

Axiom® Genome-Wide CEU 1 Array Plate (pdf, 859 KB)

Axiom® Genome-Wide CHB 1 Array Plate (pdf, 848 KB)

Axiom® Genome-Wide PanAFR Array Plate Set (pdf, 1.09 MB)

Axiom Array Plates

Part #	Description	Details
901591	Axiom™ Genome-Wide Human Array Plates	Includes 1 96-array plates and required GeneTitan plastic consumables
901592	Axiom™ Genome-Wide Human Array Plates	Includes 2 96-array plates and required GeneTitan plastic consumables
901596	Axiom™ Genome-Wide Human Array Plates	Includes 8 96-array plates and required GeneTitan plastic consumables

Axiom Reagent Kits

Part #	Description	Details
901281	Axiom™ Reagent Kit*	Includes all reagents (except isopropanol) to process 96 gDNA samples *Reagent kits do not include plastic consumables required to run the assay on the Beckman Biomek FX^P Target Prep Express System. For a list of required Beckman consumables, please see the Axiom User Guide.

Instrumentation

Part #	Description	Details
A83103	Beckman Biomek® FX^P Target Prep Express System	Robotic platform for automated target preparation

Related Products

Part #	Description	Details
	GeneTitan™ Multi-Channel (MC) Instrument	See product page for more information
	Genotyping Console™ Software 4.0	See product page for more information
000740	Axiom™ Genotyping Services	Sample processing and data generation services for gDNA samples (minimum 1,000 samples) using the Axiom Genotyping Solution