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Gene 1.0 ST Array Data Set
Chip
Sample
Set
AGCC ARR/CEL
AGCC ARR/CEL/CHP
GCOS Flat Files
Human Gene 1.0 ST Array
Tissues
Download
(131 MB)
Download
(134 MB)
Download
(129 MB, contents)
Human Gene 1.0 ST Array
Tissue Mixture
Download
(134 MB)
Download
(136 MB)
Download
(132 MB, contents)
Human Exon 1.0 ST Array
Tissue Mixture
Download
(745 MB)
Download
(716 MB)
Download
(717 MB, contents)
Human Genome U133
Plus 2.0 Array
Tissue Mixture
Download
(151 MB)
Download
(157 MB)
Download
(146 MB, contents)
Mouse Gene 1.0 ST Array
Tissues
Download
(144 MB)
Download
(147 MB)
Download
(145 MB, contents)
Rat Gene 1.0 ST
Array
Tissues
Download
(144 MB)
Download
(147 MB)
Download
(146 MB, contents)

 

DESCRIPTION OF DOWNLOADS

  • GCOS Flat Files: This zip file contains XDA format CEL files. These files are intended for use with Expression Console™ and other Gene 1.0 ST Array analysis software. They are not intended for import into GCOS systems.
  • AGCC ARR/CEL: These are zipped files converted with DEC from the GCOS Flat Files. These zip files contain richly annotated sample files (*.ARR) and AGCC-formatted probe cell intensity data (*.CEL). These data can be parsed with the Fusion SDK and are easily imported into Affymetrix Command Console or Expression Console.
  • AGCC ARR/CEL/CHP: These are zipped study files from Expression Console v1.1. These zip files contain richly annotated sample files (*.ARR), AGCC formatted probe cell intensity data (*.CEL), and AGCC-formatted probe level summarization data (*.CHP). CHP files were generated using RMA and for exon arrays using only the core probes. These data can be parsed with the Fusion SDK and are easily imported into Affymetrix® Command Console® Software or Expression Console.

DESCRIPTION OF SAMPLE SETS


Tissues:


This data set contains a collection of 11 tissues. The RNA samples are from a commercial source. Each tissue has three biological replicates; there are no technical replicates. The 11 tissues are listed below:

  Brain     Lung (Mouse, Rat)       Thyroid (Human)
  Breast (Human)     Pancreas (Human)          
  Embryo (Mouse/Rat)     Prostate          
  Heart     Skeletal Muscle          
  Kidney     Testis          
  Liver     Thymus (Mouse, Rat)          

Mixtures:

This data set contains various mixture levels of two tissues: brain and heart. A single RNA pool was created for each mixture level. Each RNA pool (mixture level) was allocated into three different target preparation replicates. Thus, each triplicate set is derived from one RNA and three (nine for the 50/50 mixture) distinct hybridization target preparations. Each mixture level has three replicates except for the 50/50 mixture level, which has nine replicates.

The mixture levels are proportioned as follows:

  Mix 1 Mix 2 Mix 3 Mix 4 Mix 5 Mix 6 Mix 7 Mix 8 Mix 9
Brain 0.00 0.05 0.10 0.25 0.50 0.75 0.90 0.95 1.00
Heart 1.00 0.95 0.90 0.75 0.50 0.25 0.10 0.05 0.00
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