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Fluidics Scripts

Fluidics scripts are protocols, or methods, used by GeneChip® Fluidics Stations for the washing and staining of GeneChip® arrays. The scripts are specific for various applications, array formats, reagents used and models of the Fluidics Station.

INSTRUCTIONS

Previously, Affymetrix provided two separate installers for fluidics scripts: The FS 400 installer and the FS 450 fluidics script installer. In an effort to streamline revisions and maintenance of fluidics scripts for both the FS450 and the FS 400 stations, Affymetrix has decoupled the fluidics script packages (that contain the actual scripts) from the fluidic script installer. The single installer can be used with GCOS or AGCC to install fluidic scripts located in the packages. The Fluidics Script Packages and Fluidics Script Installer are available to download from this web site.

During the installation process, you will be able to choose which fluidics scripts you want to install.

DOWNLOADS
   


Affymetrix login will be required.

Fluidics Installer: Please download the fluidics installer and extract to a folder using the unzip application. The fluidics installer can be used for installing protocols on GCOS or AGCC. After downloading the installer, start the installer executable (FluidicsScriptInstaller.exe) and point to the fluidic script package. The installer executable can be used for installing protocols on Affymetrix GeneChip® Command Console® (AGCC) or GeneChip Operating Software (GCOS). Please note WinZip® requirements listed at the bottom of the page.

Fluidics Installer for AGCC or GCOS (zip, 352 KB)

Fluidics Script Packages: Please download the fluidics package for the appropriate fluidics station. The fluidics scripts can be installed on the workstation by pointing the fluidics installer to the fluidics package (ZIP) downloaded from the web. It is not necessary to unzip the fluidics script package.

Affymetrix Fluidics Script Package
Fluidics Station 400 Package (zip, 160 KB) Last updated 22 Oct 2007
Affymetrix Fluidics Script Package
Fluidics Station 450 Package (zip, 324 KB) Last updated 22 Oct 2007
COMPATIBLE FLUIDIC SCRIPTS
   

Please use the compatible fluidics script application below to determine which fluidics script is appropriate for your application. Alternately, please refer to the tables in the Fluidics Script Compatible Table (pdf, 146 KB). You may need to reference some specific information regarding your application to determine the appropriate fluidics script, such as:

  • The GeneChip® array type and format used
  • The specific reagents used (e.g., for 3' Eukaryotic arrays: the GeneChip® IVT Labeling Kit vs. other labeling kits; the GeneChip® Hybridization, Wash, and Stain Kit vs. user-prepared solutions)
  • The specific GeneChip® Fluidics Station used

Please also refer to the GeneChip® array and reagent Package Inserts and Technical Manuals for additional information on fluidics scripts.

Affymetrix GeneChip® Compatible Fluidics Script Application: The compatible script for all commercial arrays can be identified by populating the drop-down lists below and then selecting the appropriate fluidics station.

Step 1: Select Design Type
Step 2: Select Probe Array Type
Step 3: Select Labeling Kit (if applicable)
Step 4: Select Hybridization, Wash and Stain Procedure
Step 5: Select Fluidics Station Model
Fluidics Script to Use
   

Affymetrix GeneChip® Compatible Fluidics Script Table: The compatible script for the Affymetrix GeneChip® arrays can be identified from the fluidics script table in the PDF file.

Fluidics Script Compatible Table (PDF, 146 KB) Last updated on 24 Aug 2007

WinZip® is required for installation. Download and install a free evaluation version of WinZip. Then, download the library file using the link below and double click on the downloaded file.

If the WinZip Wizard interface is used, simply follow the on-screen instructions for decompressing and installing the files.

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POPULAR DOWNLOADS
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Manual, Expression Analysis Technical Manual
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