Whole-transcript (WT) Sense Target Assay for use with GeneChip® Exon and Gene Array Designs
The Affymetrix® Whole-transcript Sense Target Labeling Assay is designed to generate unbiased, amplified and biotinylated sense-strand DNA targets from the entire expressed genome. Using the Whole-transcript Assay in combination with GeneChip® Exon 1.0 ST and Gene 1.0 ST Arrays allows you to measure expression across the entire length of a gene, thus offering a more complete and accurate picture of overall gene expression than 3'-based assays.
Related Content
- Genechip Exon and Gene Array System
- Whole Transcript (WT) Sense Target Labeling Manual (pdf, 795 KB)
- Whole Transcript (WT) Expression Analysis Application Note (pdf, 562 KB)
The training covers the following sections:
- Exon and Gene-level Array Designs
- Advantages of the WT Assay
- WT Assay Workflow
- Ribosomal RNA Removal for Exon Arrays
- WT Assay Protocol Steps
- Assay Quality Metrics and Performance
View the WT Assay Tutorial (33:00)