Mapping 500K Assay

Mapping 500K Assay - Online Training

These Affymetrix® Mapping 500K Assay tutorials describe how to run the 500K assay protocol to achieve higher call rates. This presentation provides a detailed overview of the steps included in the 500K manual, and covers key topics such as equipment and calibration, reagents, consumables, sample processing, operational management, workflow, ramp-up and support.

Related Content

 

The accompanying videos contain information on key stages of the 500K assay, including: Overview, Equipment/Calibration, Reagents, Consumables, Sample Processing, Operational Management, Workflow, Ramp-Up and Support.

Introduction (15:00)

Windows Media Player | QuickTime

 

Stage 1: DNA Preparation (3:00)

Windows Media Player | QuickTime

 

Stage 2: Digestion (7:00)

Windows Media Player | QuickTime

 

Stage 3: Ligation (6:00)

Windows Media Player | QuickTime

 

Stage 4: PCR (11:00)

Windows Media Player | QuickTime

 

Stage 5: Purification and Elution (9:00)

Windows Media Player | QuickTime

 

Stage 6: Quantitation/Normalization (11:00)

Windows Media Player | QuickTime

 

Stage 7: Fragmentation (9:00)

Windows Media Player | QuickTime

 

Stage 8: Labeling (5:00)

Windows Media Player | QuickTime

 

Stage 9: Hybridization (13:00)

Windows Media Player | QuickTime

 

Stage 10: Quality Control (6:00)

Windows Media Player | QuickTime

 

Stage 11: Review (2:00)

Windows Media Player | QuickTime