These Affymetrix® Mapping 500K Assay tutorials describe how to run the 500K assay protocol to achieve higher call rates. This presentation provides a detailed overview of the steps included in the 500K manual, and covers key topics such as equipment and calibration, reagents, consumables, sample processing, operational management, workflow, ramp-up and support.
Related Content
- Genotyping Console™ Software
- The Affymetrix Mapping 500K Array Set
- Affymetrix Microarray Bulletin Association Articles
The accompanying videos contain information on key stages of the 500K assay, including: Overview, Equipment/Calibration, Reagents, Consumables, Sample Processing, Operational Management, Workflow, Ramp-Up and Support.
Introduction (15:00)
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Stage 1: DNA Preparation (3:00)
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Stage 2: Digestion (7:00)
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Stage 3: Ligation (6:00)
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Stage 4: PCR (11:00)
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Stage 5: Purification and Elution (9:00)
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Stage 6: Quantitation/Normalization (11:00)
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Stage 7: Fragmentation (9:00)
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Stage 8: Labeling (5:00)
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Stage 9: Hybridization (13:00)
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Stage 10: Quality Control (6:00)
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Stage 11: Review (2:00)
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