Mapping 500K Assay Tutorials
These Affymetrix® Mapping 500K Assay tutorials describe how to run the 500K assay protocol to achieve higher call rates. This presentation provides a detailed overview of the steps included in the 500K manual, and covers key topics such as equipment and calibration, reagents, consumables, sample processing, operational management, workflow, ramp-up and support.
Mapping 500K Assay Tutorials
The accompanying videos contain information on key stages of the 500K assay, including: Overview, Equipment/Calibration, Reagents, Consumables, Sample Processing, Operational Management, Workflow, Ramp-Up and Support.
Introduction - (15:14)
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Stage 1: DNA Preparation (2:48)
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Includes: Detailed Description
Stage 2: Digestion (7:37)
Windows Media Player | QuickTime
Includes: Detailed Description
Stage 3: Ligation (6:22)
Windows Media Player | QuickTime
Stage 4: PCR (10:46)
Windows Media Player | QuickTime
Stage 5: Purification and Elution (9:33)
Windows Media Player | QuickTime
Stage 6: Quantitation/Normalization (10:56)
Windows Media Player | QuickTime
Stage 7: Fragmentation (8:53)
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Stage 8: Labeling (4:47)
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Stage 9: Hybridization (13:23)
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Stage 10: Quality Control (6:23)
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Stage 11: Review (2:17)
Windows Media Player | QuickTime
Related Content
- Genotyping Console™ Software
- The Affymetrix Mapping 500K Array Set
- Affymetrix Microarray Bulletin Association Articles
