Affymetrix University - Expression Data Analysis Course

During the Affymetrix University Expression Data Analysis Course, attendees are guided through the entire analysis pipeline of an expression experiment, from initial design to final biological interpretation, using industry-leading software solutions and peer-reviewed analysis methodology. Theoretical presentations are backed with hands-on practical exercises and interactive roundtable discussions. Class sizes are small, limited to 20 participants, to encourage interaction and to ensure focused instruction. Training assumes only basic experience with microarray analysis and utilizes a focused curriculum that enables attendees to effectively and appropriately analyze their expression experiments.

Affymetrix University Data Analysis courses are ideal if you are:

• A new or intermediate microarray user
• New to data analysis or have limited experience
• Designing an expression study and would like guidance on how to properly design the experiment
• Interested in learning more about the Affymetrix platform

Expression Data Analysis - Topics and Exercises

• Exclusive - Participants will have open access to software experts from Partek and Ariadne.
• Expression analysis overview – Review the basics of expression analysis on the Affymetrix 3' IVT, whole–transcript, and miRNA platforms.
• Experiment design - Learn how to plan effective and efficient experiments to lower costs, reduce noise, and ensure productive data analysis.
• Probe set summarization and normalization - Review available methods and trends in probe set summarization and normalization.
• Quality control - Explore available methods and guidelines for QC, a critical component of successful microarray expression studies.
• Microarray statistics 101 - Learn to correctly apply appropriate statistical methods and corrections used in peer-reviewed studies.
• Dealing with noise and unwanted variability - Examine ANOVA-based methods to remove batch effects and improve detection of true differentials.
• Statistical analysis and filtering of expression data - Perform in-depth exercises to demonstrate the application of different statistical and filtering procedures.
• Clustering and data visualization - Learn various visualization techniques for expression data and ways to explore the similarity of expression profiles and experimental samples.
• Pathway analysis - Construct network diagrams to elucidate interactions among lists of interesting genes.
• Biological interpretation - Investigate molecular mechanisms and biological processes that are implicated by annotation and literature.
• Considerations for publishing - Learn about standards for public database submission and recommendations for peer-reviewed studies.
• Social networking event - Discuss your work with colleagues in an informal setting during an evening event for all participants.

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Affymetrix University Expression Data Analysis uses Partek® Genomics Suite™ and Ariadne Pathway Studio® Explore, Affymetrix Edition, for hands-on downstream analysis. Short-term software licenses to both packages are included with registration, so participants can apply methods learned at Affymetrix University to their own studies and data sets after the course.

Course registrants will receive a 90-day license to Partek Genomics Suite™. Partek Genomics Suite is a comprehensive suite of advanced statistics and interactive data visualization tools specifically designed to reliably extract biological signals from a variety of genomic data sets in a fast and memory-efficient manner. Designed for high-dimensional genomic studies containing thousands of samples, Partek GS will analyze large data sets on a personal computer.

Course registrants will receive a 30-day license to Ariadne Pathway Studio® Explore, Affymetrix Edition. Pathway Studio® Explore, Affymetrix Edition enables pathway analysis of the entire probe list, associating genes into like–functioned or like–associated biological properties using advanced data analysis algorithms. Pathway analysis adds a biological perspective to your experimental data and takes your understanding beyond clustering comparisons and heat–map visualization.

Registration

Registration for this course includes:

• The use of a laptop for the session
• All course materials
• 90-day license for Partek Genomics Suite™
• 30-day license for Ariadne Pathway Studio® Explore, Affymetrix Edition
• Breakfast, lunch, and refreshments on each day
• Participation in the social networking dinner

Register for the Affymetrix University Expression Data Analysis course using the forms below.

To find out more about course availability, and get details on discounted group rates and hosting on-site training sessions, please contact us.

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