WT Sense Target Labeling Assay
Frequently Asked Questions
- What is the basic principle of the ribosomal RNA reduction procedure using the RiboMinus Human/Mouse Transcriptome Isolation Kit?
- Why is Betaine added to the RiboMinus Hybridization Buffer?
- Why do you choose to use an rRNA-reduction strategy but not a poly-A mRNA-specific selection protocol?
- What is the recommendation on how the total RNA samples should be prepared for this assay?
- Does genomic DNA contamination in the sample interfere with the results, and how do I monitor the degree of its effect?
- What starting material is needed for the assay?
- What is the typical cRNA yield after the IVT reaction in the first cycle?
- What is the basic principle of the single-stranded DNA fragmentation and labeling procedure?
- What is the basic component in the DNA Labeling Reagent?
- What is the expected length of the fragmented DNA target?
- Are there any safe stopping points in the assay?
- How much single-stranded DNA target do you need to hybridize to one array?
- What is the hybridization condition?
- Can I hybridize the DNA target to the HG-U133 arrays?
- Can I use this protocol for prokaryotic arrays?
- How does this protocol perform on partially degraded samples?
- Why is there no pre-hybridization step for the arrays using the targets from the WT Assay?
- What Fluidics Protocol do I use for the GeneChip® Human Exon Array?
- How long does it take to scan an array?
- What are the internal grid lines on the array image for?
- How do I check for the correct gridding of the new array?