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WT Sense Target Labeling Assay
Does genomic DNA contamination in the sample interfere with the results, and how do I monitor the degree of its effect?
By titrating genomic DNA back into the total RNA samples and monitoring the deterioration of the array data, it was determined during development of the assay that a moderate amount of genomic DNA contamination will only have minimum effect on the array results. Therefore, routine RNA isolation techniques coupled with DNase treatment should yield sufficiently high-quality sample for analysis on the exon arrays.