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WT Sense Target Labeling Assay
Why do you choose to use an rRNA-reduction strategy but not a poly-A mRNA-specific selection protocol?
It has been shown that a portion of the transcripts in total RNA do not necessarily contain Poly-A tails; therefore, they will be excluded by a poly-A RNA positive selection technique. The rRNA reduction approach will also make the protocol more robust in handling smaller amounts of total RNA samples as little as 1 μg.