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WT Sense Target Labeling Assay
Why is there no pre-hybridization step for the arrays using the targets from the WT Assay?
The pre-hybridization step was required for the 3' target in the GeneChip® Expression Analysis Technical Manual.
No pre-hybridization step is necessary for the WT targets. There are
many differences between the WT targets and the 3' targets in terms
of the nature of the molecules (DNA vs. RNA), as well as labeling
molecule and hybridization cocktail makeup. It has been found that
pre-hybridization is not necessary for the WT targets.