WT Sense Target Labeling Assay

Are there any safe stopping points in the assay?

There are a few safe stopping points in the assay, including:

After IVT reaction and the cRNA cleanup step in the first cycle, before proceeding to the second cycle of reverse transcription
After reverse transcription and the single-stranded cDNA cleanup step in the second cycle, before fragmentation and labeling
After fragmentation and labeling, before hybridization