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In Microarray Suite, click on the tools button on the menu bar. Select Analysis Settings, then Expression to open a dialog box. Chose the Rat 230A or Rat 230B probe array type from the dropdown list at the top of the dialog box. Now, select the Scaling tab. Click the radio button labeled Selected Probe Sets. To create a normalization mask file using a subset of these normalization genes, click Create/Open button. Give the new mask file a name and click open. Next, select the probe sets to use in the normalization from the exclude list on the left and once highlighted click the include button to move them to the include list on the right. Then click OK to return to the Expression Analysis Settings dialog box. Now you can use the browse button to select the mask file to use in the normalization and set the target intensity for your analysis. |