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GeneChip® Rat Expression Set 230
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How were the normalization controls determined?
A candidate list of probe sets was obtained in collaboration with an external site based on a large number of tissue samples run on RG-U34 arrays. Criteria for selection included evidence of constitutive expression (detection in at least 95% of replicates) and minimal variation in signal across the tissues tested. The 11 probe pair probe sets for these transcripts were evaluated by hybridizing 11 tissues and 4 cell lines on Rat 230A and B arrays. One hundred probe sets were selected that had detection in at least 95% of replicates and had minimal signal variation. Preference was given to RefSeq sequences where possible, probe sets were selected to give a range of signal values and to represent 100 unique UniGene clusters.