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The Baylor College of Medicine Human Genome Sequencing Center (June 2002) assembly was used to design the Rat Set 230. Specifically, the genomic sequence was used for trimming low quality portions of EST sequencing reads and for improving orientation calls through the use for flanking intronic sequence. The probe sets themselves are constructed against the consensus sequence for cDNA sequence clusters. The Rat Set 230 is not a genome tiling product, but rather an mRNA expression product; thus the entire rat genomic sequence is not represented. |