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Probe Sequence

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What is contained in the tab-delimited format of the probe sequence download file?

The probe sequence download file in the Download Center is a tab-delimited file containing the following columns:

Probe Set Name: For example, 1007_s_at
Probe X: The X coordinate of the probe sequence on the GeneChip® probe array.
Probe Y: The Y coordinate of the probe sequence on the GeneChip probe array.
Probe Interrogation Position: The position of the 13th ("middle") nucleotide of the probe sequence as it aligns on the consensus/exemplar sequence.
Probe Sequence: The 25-base perfect match sequence.
Target Strandedness: The sense/antisense orientation of the target sequence that can hybridize with the probe sequence.

Note: Probe Sequence databanks in the NetAffx™ Analysis Center have an additional column called, "Serial Order". This column provides the relative order of probe sequences as they align with the consensus/exemplar sequence.