When should I select the "Search With Reverse Complement of Query" option in the Probe Match tool?
The probe sequences on the anti-sense arrays are designed to hybridize with the anti-sense strand of the corresponding gene sequence. This means that all the probe sequences are in the sense orientation. Select the "Search With Reverse Complement of Query" option if you do not obtain any matches (or "hits"), with the query sequence or if you are not sure about the orientation of your query (input) sequence. Please note: Use caution when interpreting results from such a query to avoid selecting false positive "hits" to your gene of interest.