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GeneChip® Eukaryotic Poly-A RNA Control Kit

Frequently Asked Questions

  1. What is this kit used for?

  2. What components are in each kit?

  3. What are the assumptions you have used in estimating the concentrations in terms of complexity ratio of these control transcripts in a sample?

  4. Based on these assumptions, how did you calculate the concentrations in terms of complexity ratios from the starting concentrations of the poly-A RNA controls in the Poly-A Control Stock?

  5. How many reactions does each kit contain?

  6. Where can I find the protocol for diluting the Poly-A Control Stock before adding to the samples?

  7. At what temperature should the kit be stored?

  8. What is the shelf life of the kit?

  9. How do I evaluate the sensitivity and efficiency of the labeling reaction based on the Poly-A Controls?

  10. Should I anticipate the same results using the Poly-A controls with all eukaryotic GeneChip® expression arrays?

  11. How does this kit perform with labeling kits other than those recommended in the Affymetrix protocols?

  12. Can this kit be used with prokaryotic samples and arrays?

  13. Can I use the signal intensities of the Poly-A controls as standard curve for transcript abundance quantitation?

  14. The Poly-A Control Dil Buffer comes in one big bottle of 3.8 mL and it is inconvenient to freeze/thaw each time since it freezes when stored at -20°C. What do you recommend and can you change the packaging so the buffer comes in smaller aliquots?

  15. What is in the Poly-A Control Dil Buffer?

  16. Do you have any specific recommendation on the type of non-stick tubes to use for diluting the Poly-A RNA Control Stock?

  17. Can I purchase the Poly-A Control Stock and the Poly-A Control Dil Buffer separately?

  18. Can I purchase one, or all, of the four Poly-A Controls separately?