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Depending on the quantity of the starting material and if the poly-A controls are used in the Standard Assay or the Small Sample Target Labeling Assay vII, the amount of poly-A controls used in each sample will vary, causing the number of reactions contained in the Kit to vary as well. In addition, it is related to the number of samples being processed at the same time since the Poly-A Control Stock needs to be diluted serially before adding to the sample. The intermediate dilutions should be prepared fresh each time and, therefore, depending on the number of samples to be labeled at the same time, the amount of Poly-A Control Stock used may vary.
Our rough estimation is that each Kit is sufficient for approximately 100 reactions. |