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GeneChip® Eukaryotic Poly-A RNA Control Kit

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What are the assumptions you have used in estimating the concentrations in terms of complexity ratio of these control transcripts in a sample?

The concentrations in terms of complexity ratios are calculated based on the following assumptions:

  • Average transcript length = 2,000 bases
  • Average MW of a single base = 330 g/mole
  • mRNA constitutes approximately 2% of the total RNA sample