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Affymetrix GeneChip® Eukaryotic Poly-A RNA Control Kit
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What are the assumptions you have used in estimating the concentrations in terms of complexity ratio of these control transcripts in a sample?
The concentrations in terms of complexity ratios are calculated based on the following assumptions:

•Average transcript length = 2,000 bases
•Average MW of a single base = 330 g/mole
•mRNA constitutes approximately 2% of the total RNA sample