search help
Back Next Glossary All Topics
Affymetrix GeneChip® Eukaryotic Poly-A RNA Control Kit
index previous question next question
What components are in each kit?
Four independent poly-A RNA controls are provided conveniently in a pre-mixed stock solution at staggered concentrations. After spiking directly into eukaryotic RNA samples, labeled cRNA targets are prepared and hybridized onto GeneChip expression arrays. The resultant Signal intensities for the poly-A RNA controls serve as sensitive indicators of the efficiency of the labeling reaction.

Poly-A RNA Controls Final Concentration* in Samples
lys 1:100,000
phe 1:50,000
thr 1:25,000
dap 1:7,500

*Concentrations are defined as the number of copies of a particular poly-A RNA control to the total number of poly-A RNA molecules in the complex sample.

Volume Components
16 µL Poly-A Control Stock
3.8 mL Poly-A Control Dil Buffer