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This product was designed and tested for eukaryotic arrays. The presence of poly-A tails on the transcripts is not relevant to prokaryotic samples. Since the assays are fundamentally different, this Kit may not provide similar array results with prokaryotic assays and has not been tested at Affymetrix for this application.
Probe sets for all four controls are available on prokaryotic arrays including the E. coli (antisense) and P. aeruginosa arrays. Adding the Poly-A RNA Controls to these samples will theoretically also serve as a valuable positive control. However, customers who are interested in using the controls for prokaryotic applications will need to optimize the spike-in ratios to obtain the best range for evaluating both assay sensitivity and dynamic range. Affymetrix does not currently provide support on the Eukaryotic Poly-A RNA Control Kit when used in prokaryotic assays. |