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GeneChip® Eukaryotic Poly-A RNA Control Kit
Should I anticipate the same results using the Poly-A controls with all eukaryotic GeneChip® expression arrays?
The Signal intensity representative data (globally scaled) shown in the package insert are typical results for the newest probe design from Affymetrix (3' AFFX-r2-Bs). These probe sets are available on catalog arrays including the GeneChip® Human Genome U133, Mouse 430, and Rat 230 arrays.
However, with previous generations of probe sets, the Signal values observed may vary from those shown. Nevertheless, utilization of these controls still serves as a good indicator of the experimental process quality, low-end sensitivity, and assay dynamic range. Some of the arrays that have the older probe sets include the Drosophila Genome Array and Yeast Genome S98 Array.