| What is the nature of specificity of the probe sets included in the GeneChip Mouse Expression Set 430? | |||
| The probes on the Mouse Set 430 are designed to detect the anti-sense strand of the gene of interest, and these probe sets are annotated with the "_at" extension. The primary goal in probe selection is to select a probe set unique to a single transcript or common among multiple transcripts from the same gene.
Often it is not possible to select a probe set that is unique to a single transcript because multiple transcript variants share common sequence. Such probe sets have the "_s" extension. Occasionally, it is not possible to select a unique or shared probe set. Such probe sets have the "_x" extension. For more information, please review the "_s" and "_x" probe set descriptions. Please note: There are no "_f", "_g", "_r", "_i", or "_n" probe sets in the Mouse Expression Set 430 design. This is because the non-unique probe set types were simplified and adjusted to account for improvements to the probe selection rules. |
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