Why do I need to use the 20X Hybridization Controls?
The Hybridization controls are high-quality controls for monitoring array hybridization, washing, and staining for reproducible results.
20X Hybridization Controls are composed of a mixture of biotinylated and fragmented cRNA of bioB, bioC, and bioD from E. coli, and cre from P1 bacteriophage in staggered concentrations. The premixed controls are ready to be added directly to the hybridization cocktail. Probes detecting these controls are present on the GeneChip® miRNA Array.
The 20X Hybridization Controls are spiked into the hybridization cocktail, independent of RNA sample preparation, and are thus used to evaluate sample hybridization efficiency on eukaryotic gene expression arrays.
As the 20X Hybridization Controls are used by Affymetrix to troubleshoot potential array and array processing issues, a failure to include the 20X Hybridization Controls in the hybridization cocktail will void the customer's ability to submit an array replacement request to Affymetrix.