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3'-Amplification Reagents for IVT Labeling

Frequently Asked Questions

  1. What does the GeneChip® IVT Labeling Kit contain?

  2. Why are you recommending the overnight incubation for the IVT reaction?

  3. Should I be concerned about getting shorter cRNA targets because of the overnight IVT incubation?

  4. Does this IVT Labeling Kit work for both the One- and Two-Cycle Target Labeling protocols?

  5. What is the typical cRNA yield of the IVT Labeling Kit?

  6. How do I know if the kit is working well without hybridizing my targets to GeneChip arrays?

  7. How do the labels in the GeneChip IVT Labeling Kit work?

  8. The recommended starting amount of total RNA for the One-Cycle protocol has been reduced from 5 µg to 1 µg. Do you have any data to support that the procedure works well with the reduced amount of starting material?

  9. Can I compare GeneChip array data from samples labeled with Enzo to those labeled with the new GeneChip IVT Labeling Kit?

  10. Why have you modified the hybridization cocktail composition by including 10% DMSO and changed the Fluidics Scripts?

  11. How can I get the modified Fluidics Scripts that are compatible with the new kit?

  12. Can I use the new IVT Labeling Kit with the previous version of the Eukaryotic Hybridization Controls (P/N 900299 and 900362)?

  13. What is the storage condition of the IVT Labeling Kit?

  14. What end user license will be provided with the new Labeling Reagent?