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			The recommended starting amount of total RNA for the One-Cycle protocol has been reduced from 5 µg to 1 µg. Do you have any data to support that the procedure works well with the reduced amount of starting material?
			Studies were conducted at Affymetrix to compare the data obtained from 1 µg vs. 5 µg of starting total RNA, and the array results were highly concordant with respect to detection sensitivity (percent Present call) and reproducibility (Signal correlation, Absolute Call concordance). However, subtle differences were observed. Therefore, for best comparability of results, it is generally recommended to use the same amount of starting RNA in studies where the data are intended to be directly compared.