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3'-Amplification Reagents for IVT Labeling
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Should I be concerned about getting shorter cRNA targets because of the overnight IVT incubation?
It has been shown using electrophoresis that the length of the cRNA products does not change with increasing IVT reaction time. In addition, array results generated after varying the length of IVT incubation are highly concordant with shorter incubation times and show little or no detectable difference.