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3'-Amplification Reagents for IVT Labeling
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What does the GeneChip® IVT Labeling Kit contain?
The IVT Labeling Kit contains all necessary reagents (see the following table) to perform thirty in vitro transcription (IVT) reactions from double-stranded cDNA templates. The template cDNA containing the T7 promoter sequence can be obtained following either the One- or Two-Cycle cDNA Synthesis protocols.
| Component Name | Quantity | Volume |
|---|---|---|
| 10X IVT Labeling Buffer | 1 |
120 µL |
| IVT Labeling Enzyme Mix | 1 |
120 µL |
| IVT Labeling NTP Mix | 1 |
360 µL |
| 3'-Labeling Control (0.5 µg/µL) | 1 |
10 µL |
| RNase-free Water | 1 |
910 µL |