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3'-Amplification Reagents for IVT Labeling
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What does the GeneChip® IVT Labeling Kit contain?
The IVT Labeling Kit contains all necessary reagents (see the following table) to perform thirty in vitro transcription (IVT) reactions from double-stranded cDNA templates. The template cDNA containing the T7 promoter sequence can be obtained following either the One- or Two-Cycle cDNA Synthesis protocols.

Compnent name Quantity Volume

10X IVT Labeling Buffer

1 120 µL
IVT Labeling Enzyme Mix 1 120 µL
IVT Labeling NTP Mix 1 360 µL
3'-Labeling Control (0.5 µg/µL) 1 10 µL
RNase-free Water 1 910 µL