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GeneChip® Human Genome U133 2.0 Arrays

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Why are the following probe sets on the HG-U133A and HG-U133B Arrays but are not on the HG-U133 Plus 2.0 and HG-U133A 2.0 Arrays?

AFFX-r2-Hs18SrRNA-5_at
AFFX-r2-Hs18SrRNA-M_x_at
AFFX-r2-Hs18SrRNA-3_s_at
AFFX-r2-Hs28SrRNA-5_at
AFFX-r2-Hs28SrRNA-M_at
AFFX-r2-Hs28SrRNA-3_at

The AFFX-r2-Hs18SrRNA and AFFX-r2-Hs28SrRNA probe sets (3', M and 5') were not included in the HG-U133 A 2.0 and HG-U133 Plus 2.0 array designs though they were included in the HG-U133 Set (A and B). The probe selection regions for 18SrRNA r1 and r2 probe sets are highly overlapping and have performed similarly in internal testing. There is less overlap between the r1 and r2 28SrRNA probe selection regions. Users who are interested in evaluating ribosomal RNA probe sets should use the AFFX-r1-Hs18SrRNA probe sets (3', M, and 5').