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At the time of the HG-U133 Set design the "_a" probe set was not defined. The non-unique probe set type, "_a", was introduced with the Mouse Expression Set 430 to indicate probe sets that recognize multiple alternative transcripts from the same gene. Probe sets with common probes among multiple transcripts from separate genes are annotated with a "_s" suffix. Another way to describe this is that "_a" probe sets have a gene cluster count = 1 and transcript count >1, and "_s" probe sets have a gene cluster count > 1 and transcript count >1. The gene cluster count and transcript counts are available through the NetAffx™ Analysis Center. For consistency, the names of existing probe sets with the "_s" suffices were not changed between the HG-U133 Set and the HG-U133 Plus 2.0 and HG-U133A 2.0 Arrays. The new content on the HG-U133 Plus 2.0 Array incorporates both the "_a" and "_s" probe sets. |