What are the major improvements in the design of the HG-U133 Arrays?
The GeneChip® Human Genome U133 Set incorporates significant advances in array design. The resulting two-array set is a powerful tool, providing you the best view of the human transcriptome.
Design features include:
- Using genomic sequences to verify sequence selection, orientation, and the quality of sequence clustering.
- Using clustering information from UniGene (Build 133), in combination with primary sequence and annotation information from a number of public databases, to give a more complete and accurate array design data set.
- Using probe selection methods that use a multiple linear regression model, derived from a thermodynamic model of nucleic acid duplex formation, which predicts probe binding affinity and linear signal response to varying target concentrations.
- Leveraging feature size reduction from 20 to 18 microns to allow for more probe sets per array, with no reduction in performance.
For more information on array design and probe selection of the HG-U133 Array, see the Array Design for the GeneChip® Human Genome U133 Set Technical Note.