Genome-Wide Human SNP Array 5.0

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Total genomic DNA (500ng) is digested with Nsp I and Sty I restriction enzymes and ligated to adaptors that recognize the cohesive four basepair (bp) overhangs. All fragments resulting from restriction enzyme digestion, regardless of size, are substrates for adaptor ligation. A generic primer that recognizes the adaptor sequence is used to amplify adaptor-ligated DNA fragments. PCR conditions have been optimized to preferentially amplify fragments in the 200 to 1,100 bp size range. PCR amplification products for each restriction enzyme digest are combined and purified using activated beads. The amplified DNA is then fragmented, labeled, and hybridized to a Genome-Wide Human SNP 5.0 Array.