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Gene Expression Assay and Data Analysis
What are the different target labeling assays for GeneChip® expression arrays?
The majority of commercial GeneChip® expression arrays are antisense arrays - they interrogate targets that are prepared in the antisense orientation. The E.coli Genome Array is an exception; it was designed in the sense orientation. Therefore, there are three basic types of expression assays:
- cRNA target amplification and labeling assay - Used for all eukaryotic GeneChip expression arrays.
- Direct labeling of enriched RNA: used specifically for E.coli Genome Array
- cDNA target labeling assay: used for other prokaryotic GeneChip expression arrays