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The standard gene expression hybridization time is 14-16 hours at 45° C. At high
temperatures and longer incubation times the sample will evaporate. Loss of sample is undesirable for several reasons:
1. Low volume of hybridization solution in the probe array can lead to dry spots that will show up as uneven hybridization and thus, compromise data.
2. Sample loss compromises the possibility of repeating the experiment with the identical sample.
3. Sample evaporation can lead to changes in the salt concentration of the solution which can affect the stringency conditions for hybridization. |