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Highly concordant results have been obtained during our product development process by comparing global array hybridization results obtained from samples cleaned up with both protocols. The concordance was determined based on the overall signal intensity, as well as the qualitative calls. However, due to the different mechanisms associated with each cleanup procedure, there will be minor differences in the data obtained. For example, cDNA cleanup column reduces the recovery of fragments of 100 nucleotides or less, whereas these fragments are retained in the phenol/chloroform method. However, we do believe these differences are minor in magnitude. Customers are encouraged to perform their own comparisons and analysis to determine when to adopt the Sample Cleanup Module into their laboratories. |