Which controls are included in the new array?
A variety of controls are included on the Exon Array, designed specifically to facilitate the application of genome-wide exon-level expression profiling. These controls include:
- Intron controls — for approximately 100 genes with relatively constitutive expression, both exon-based and intron-based probe sets were tiled. The intron/exon normalization control probe sets can be used to monitor contamination from genomic DNA, hnRNA, as well as to provide a baseline for experiment quality control.
- Hybridization controls — bioB, vbioC, bioD and cre
- Poly-A RNA controls — lys, dap, phe, and thr