 |
|
|
Insertions: If you are looking for a previously characterized insertion we can design probes to encode for known insertions of any size. Reductions in signal may signify the presence of an unknown insertion but this pattern may vary depending on the length of insertion and, therefore, be difficult to predict.
Deletions: Detecting known deletions of >25 bases is possible by image analysis of signal intensity reduction and/or no-calls. However, the current commercial software will not call deletions automatically. The current software will make No calls and customers will need to analyze the data further. Homozygous deletions will be the easiest to detect, however, one must check to make sure the No calls are due to an actual deletion rather than by a PCR failure. Heterozygous deletions will require additional bioinformatics to quantify the signal indicating a deletion. This is a potential area for automated analysis in future releases, but not available at present.
|