CustomSeq Resequencing Array
Frequently Asked Questions
- How many bases can I resequence on a CustomSeq array?
- Do I need to resequence one long region or may I also use this to sequence several shorter ones?
- How many probes/features per base?
- How many arrays do I need to order?
- What is the turn around time for a new CustomSeq array design?
- How long does an experiment take?
- What Affymetrix instrumentation, hardware and software is required to run the CustomSeq assay?
- What support materials are currently available on CustomSeq?
- What publications are currently available on CustomSeq?
- What is your overall accuracy? How does it compare to capillary sequencing?
- Do I need to manually check heterozygotes?
- What is your call rate? How does it compare to capillary sequencing?
- What is your reproducibility?
- What are the benefits of longer read-length?
- Can I detect Insertions or Deletions on a resequencing array?
- How much DNA is required?
- Can I analyze only one strand? Your chip holds 600 kb but you tile 300 kb double - stranded - can I just tile one strand and sequence a full 600 kb?
- Can the CustomSeq array detect heterozygotes?
- What does the quality score stand for?
- How does the quality score compare to Phred/ Phrap scores?
- How does CustomSeq handle GC rich regions?
- Can I use genotype for individual SNPs in many genes?
- What is the difference between GSEQ software and the ABACUS program offered through JHU?