How does CustomSeq handle GC rich regions?
We have data showing that we can achieve very high call rates (>90%) from arrays with >50% GC content. Additionally, simple modifications may be made to the assay protocol (i.e., addition of DMSO to hybridization cocktail) to further optimize for high GC content arrays. In general it is only in regions containing strings of >4 consecutive G's in a single probe that are more difficult to read due to probe hybridization characteristics.