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Two-Cycle cDNA Synthesis Kit

Frequently Asked Questions

  1. What components does the GeneChip® Two-Cycle cDNA Synthesis Kit contain?

  2. What is the shelf life of the kit?

  3. How are the kits shipped and what is the storage condition?

  4. Are the data obtained following the Small Sample Target Labeling vII (SSTLvII) comparable to those obtained using the Two-Cycle cDNA Synthesis Kit?

  5. What are the major differences and improvements comparing the new Two-Cycle cDNA Synthesis Kit with the Affymetrix® Small Sample Target Labeling Version II Protocol?

  6. Can you compare the data from the Two-Cycle protocol directly with the One-Cycle protocol?

  7. I have never performed any small sample target labeling before. Do you have any suggestions for incorporating the Two-Cycle cDNA Synthesis Kit into our routine GeneChip microarray analysis?

  8. Does this Two-Cycle cDNA Synthesis Kit/protocol work well on partially degraded RNA samples?

  9. How does your Two-Cycle cDNA Synthesis Kit protocol perform compared with Ambion's MessageAmp Kit or Arcturus's RiboAmp Kit?

  10. What is the detection sensitivity of the Two-Cycle cDNA Synthesis Kit protocol?

  11. Which RNA isolation method do you recommend for the Two-Cycle cDNA Synthesis protocol?

  12. If I have only between 100 ng and 1 μg of total RNA, which protocol would you recommend, the One-Cycle or the Two-Cycle cDNA Synthesis protocol?

  13. How do I know if my first-cycle cDNA synthesis reaction has worked well?

  14. How do I know if my first-cycle IVT reactions have worked well?

  15. In the Two-Cycle Target Labeling protocol, the first-cycle cDNA cleanup step is omitted. Is there any impact on the data by removing this step?