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If I have only between 100 ng and 1 μg of total RNA, which protocol would you recommend, the One-Cycle or the Two-Cycle cDNA Synthesis protocol? |
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Since it is recommended to choose one protocol consistently for all samples to be compared within the same study, the procedure you choose may depend on the amount of RNA obtained from the majority of the samples you plan on including in the entire study. If most of the samples fall into the 100 ng to 1 μg range, it is reasonable to use the Two-Cycle Target Labeling protocol for the whole set of experiments. |
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