Which RNA isolation method do you recommend for the Two-Cycle cDNA Synthesis protocol?
Different tissue types may have different requirements for RNA extraction. Several commercial kits and protocols are currently available for total RNA isolation from small samples (tissues, biopsies, LCM samples, etc.). Select the one that is most suitable for the processing of your samples and follow the vendor-recommended procedures closely. Assess the quality and integrity of obtained total RNA samples carefully by gel electrophoresis or Bioanalyzer before starting the GeneChip target labeling protocol.