The Two-Cycle cDNA Synthesis Kit contains all necessary reagents (see the following table) to carry out the two cycles of cDNA synthesis for thirty samples. Note that the reagents for the intermediary in vitro transcription (IVT) amplification using un-labeled ribonucleotides, between the two cDNA synthesis reactions, is not included in the kit. The Ambion MEGAscript T7 Kit is recommended.
| Component Name | Concentration | Volume | Quantity |
| T7-Oligo(dT) Primer, 50 μM | 50 μM | 120 μL | 1 |
| 5X 1st Strand Reaction Mix | 5X | 180 μL | 1 |
| DTT, 0.1M | 0.1M | 90 μL | 1 |
| dNTP, 10 mM | 10 mM | 200 μL | 1 |
| SuperScript™ II | 200 U/μL | 60 μL | 1 |
| RNase Inhibitor | 40 U/μL | 45 μL | 1 |
| 5X 2nd Strand Reaction Mix | 5X | 900 μL | 1 |
| Random Primers | 3 μg/μL | 60 μL | 1 |
| MgCl2, 1M | 1M | 60 μL | 1 |
| E.coli DNA Polymerase I | 10 U/μL | 200 μL | 1 |
| RNase H | 2 U/μL | 55 μL | 1 |
| T4 DNA Polymerase | 5 U/μL | 60 μL | 1 |
| RNase-free Water | 9.5 mL | 1 |