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Blood Sample Processing

Frequently Asked Questions

  1. Does this method work with RNA isolated from fetal blood?

  2. Can I use this method with cord blood?

  3. Does this method also work with RNA isolated from whole blood from animal samples?

  4. Does the protocol also work with RNA samples isolated from whole blood using other methods than the PAXgene Blood RNA system (e.g. Phenol)?

  5. Can the kit be used for RNA isolated from starting materials different from whole blood?

  6. Can I use methods for concentration of PAXgene RNA other than that described in the GeneChip Globin Reduction Kit Handbook?

  7. What cRNA yield can I expect if the globin reduction protocol is performed?

  8. Can I perform the protocol with less than 5 μg after concentration total RNA starting material?

  9. How can I test if blocking of cDNA synthesis is specific for globin mRNA transcripts?

  10. Why is only 2 μg of RNA used for the control reactions?

  11. How does the protocol affect the GeneChip®Array analysis?

  12. Does the globin reduction procedure affect the expression analysis of transcripts different from globin?

  13. After performing globin reduction on PAXgene Blood RNA, do I get an equivalent expression profile to the same sample when starting with RNA isolated using an erythrocyte lysis method?

  14. Does the protocol work with other Array systems?

  15. What is the effect of globin reduction on RT-PCR?

  16. Can I order my PNAs from vendors other than ABI?

  17. Why must the PNA working solutions be discarded after one use?

  18. Why do I have to incubate my PNA solutions before each use?

  19. How can I determine the concentration of a PNA solution?

  20. Can I dissolve PNA oligomers in 0.1% trifluoroacetic acid in order to increase solubility?