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GeneChip® 3' IVT Express Kit

Frequently Asked Questions

  1. Will I need to transition from the current One-Cycle Kit to the new 3' IVT Express Kit?

  2. Can I expect the same performance form the new 3' IVT Express Kit as the One-Cycle Kit?

  3. Will my 3' expression QC metrics be the same when using the new 3' IVT Express Kit as they were with the One-Cycle Kit?

  4. Can I start using the new 3' IVT Express Kit right away, even in the middle of my experiment?

  5. Can I use less than 50ng of Total RNA as input?

  6. Can I use more than 500ng of Total RNA as input?

  7. Do I have to normalize my samples prior to starting this new 3' IVT Express Kit?

  8. I notice the cDNA clean up step has been removed from this protocol. Can I also leave the cDNA clean up step out of the One-Cycle Kit?