The Annotation Converter enables you to create custom SQLite format annotations (annot.db) for use in Affymetrix® Genotyping Console software (GTC) and Affymetrix Power Tools (APT). You can customize annotations for Affymetrix® catalog genotyping arrays or generate annotations Axiom™ custom genotyping arrays such as the Affymetrix® myDesign array plates.
The steps to create custom annotations include:
apt-annotation-converter - A program for importing an annotation text files,
into the annotation database.
usage:
Import a single file:
apt-annotation-converter.exe -db-template Homo_sapiens.dbtemplate -db-file GenomeWideSNP_6.na30.annot.db -tsv-file GenomeWideSNP_6.na30.annot.csv -array-set GenomeWideSNP_6 -array-config GenomeWideSNP_6.arrayconfig
Import a list of files:
apt-annotation-converter.exe -db-template Homo_sapiens.dbtemplate -db-file GenomeWideSNP_6.na30.annot.db -tsv-files ListOfTsvToLoad.txt -array-set GenomeWideSNP_6 -array-config GenomeWideSNP_6.arrayconfig
Import using the analysis_job job file:
apt-annotation-converter.exe -xml-file Axiom_GW_Hu.analysis_job
options:
Common Options (not used by all programs)
-h, --help Display program options and extra
documentation about possible analyses. See
-explain for information about a specific
operation. [default 'false']
-v, --verbose How verbose to be with status messages 0 -
quiet, 1 - usual messages, 2 - more
messages. [default '1']
--console-off Turn off the default messages to the
console but not logging or sockets.
[default 'false']
--use-socket Host and port to print messages over in
localhost:port format [default '']
--version Display version information. [default
'false']
-f, --force Disable various checks including chip
types. Consider using --chip-type option
rather than --force. [default 'false']
--throw-exception Throw an exception rather than calling
exit() on error. Useful for debugging. This
option is intended for command line use
only. If you are wrapping an Engine and
want exceptions thrown, then you should
call Err::setThrowStatus(true) to ensure
that all Err::errAbort() calls result in an
exception. [default 'false']
--analysis-files-path Search path for analysis library files.
Will override AFFX_ANALYSIS_FILES_PATH
environment variable. [default '']
--xml-file Input parameters in XML format (Will
override command line settings). [default
'']
--temp-dir Directory for temporary files when working
off disk. Using network mounted drives is
not advised. When not set, the output
folder will be used. The defaut is
typically the output directory or the
current working directory. [default '']
-o, --out-dir Directory for output files. Defaults to
current working directory. [default '.']
--log-file The name of the log file. Generally
defaults to the program name in the out-dir
folder. [default '']
Engine Options (Not used on command line)
--command-line The command line executed. [default '']
--exec-guid The GUID for the process. [default '']
--program-name The name of the program [default '']
--program-company The company providing the program [default
'']
--program-version The version of the program [default '']
--program-cvs-id The CVS version of the program [default '']
--version-to-report The version to report in the output files.
[default '']
--free-mem-at-start How much physical memory was available when
the engine run started. [default '0']
--meta-data-info Meta data in key=value pair that will be
output in headers. [default '']
Input Options
--db-template Sqllite Annotation database template file
[default '']
--db-file Target Sqllite Annotation database file
[default '']
--tsv-file Delimited text file to import in to
Annotation table. Use either TCV or
CSV-file format [default '']
--tsv-files Text file specifying TSV files to process,
one per line [default '']
--array-config Array type specific configuration file.
[default '']
--array-set Array set name. [default '']
--message-delay Message Delay (in milliseconds) for the UI
tool. [default '']
--time-start The time the engine run was started
[default '']
--time-end The time the engine run ended [default '']
--time-run-minutes The run time in minutes. [default '']
--analysis-guid The GUID for the analysis run. [default '']
1.7.1