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GeneChip® PrimeView™ Human Gene Expression Array

Proven performance from the industry standard

PrimeView™ Human Gene Expression Array

The GeneChip® PrimeView™ Human Gene Expression Array cartridge enables expression profiling using probe sets with an emphasis on established, well-annotated content. Sequences used in the design of the array were selected from the UniGene database 219, RefSeq version 36, and full-length human mRNAs from GenBank®.

The PrimeView™ Human Gene Expression Array enables you to:

  • Measure gene expression of more than 36,000 transcripts and variants per sample
  • Get accurate and reproducible gene expression data by using multiple independent measurements for each transcript

Key benefits

  • Offers excellent gene expression accuracy and reproducibility
  • Multiple independent measurements per transcript for increased confidence in your results—11 probes per set for well-annotated sequences, 9 probes per set for the remainder
  • Provides complete coverage of the annotated genome

Content profile

  • The PrimeView™ Human Gene Expression Array is comprised of more than 530,000 probes covering more than 36,000 transcripts and variants, which represent more than 20,000 genes mapped through UniGene or via RefSeq annotation.
  • The EST and mRNA sequences used in the design were clustered and assembled to create consensus sequences that represent alternative splice forms. Each assembly was then analyzed for orientation and alternative 3' end evidence.
  • Content was chosen to cover all well-annotated genes and transcripts from RefSeq v36 and by leveraging all available EST and mRNA evidence that fall into the same clusters, to rigorously detect alternate 3' ends of those well-annotated genes.
  • More than 1,000 probe sets represent transcripts that have no official gene symbol in UniGene, but are based on predicted RefSeq sequences and UniGene clusters with good evidence of actual transcription.

For Research Use Only. Not for use in diagnostic procedures.

Manuals

3' IVT PLUS Reagent Kit User Manual (pdf, 2 MB)

Library Files

PrimeView™ Human Gene Expression Array (zip, 15.2 MB)

Package Inserts

PrimeView? Human Gene Expression Array (pdf, 128 KB)

Alignment, Annotation, and Sequence Files

Information about using Alignment, Annotation, and Sequence files

Current NetAffx Annotation Files

PrimeView Annotations, CSV format, Release 36 (38 MB, 4/13/16)

PrimeView Orthologs/Homologs, CSV Format, Release 36 (11 MB, 4/13/16)

Note: NetAffx annotation files are intended for use in automated analysis. Some are not compatible with all spreadsheet applications. Please use the NetAffx Analysis Center to limit download data to your probe sets of interest. Annotation CSV files for the exon arrays are split into a probe set-level annotation file and a transcript cluster-level annotation file; exon array CSV files are compatible with ExACT.

Sequence Files

PrimeView Consensus Sequences FASTA Format (46 MB, 8/25/11)

PrimeView Control Sequences FASTA Format (34 KB, 8/25/11)

PrimeView Probe Sequences, tabular format (8.5 MB, 8/25/11)

PrimeView Target Sequences FASTA Format (10 MB, 8/25/11)

Archived NetAffx Annotation Files

PrimeView Annotations, CSV format, Release 32 (22 MB, 8/25/11)

PrimeView Annotations, CSV format, Release 33 (26 MB, 10/30/12)

PrimeView Annotations, CSV format, Release 34 (31 MB, 1/23/14)

PrimeView Annotations, CSV format, Release 35 (33 MB, 10/7/14)

PrimeView Annotations, MAGE-ML XML Format, Release 32 (101 MB, 8/25/11)

PrimeView BLASTP annotations, CSV Format, Release 32 (2.3 KB, 8/25/11)

PrimeView Orthologs/Homologs, CSV Format, Release 32 (9.9 MB, 8/25/11)

PrimeView Orthologs/Homologs, CSV Format, Release 33 (10 MB, 10/30/12)

PrimeView Orthologs/Homologs, CSV Format, Release 34 (10 MB, 1/23/14)

PrimeView Orthologs/Homologs, CSV Format, Release 35 (11 MB, 4/16/15)

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