Scientific Publications
Most comprehensive whole-genome array set for studying protein/DNA interactions in chromatin immunoprecipitation (ChIP) experiments.
- High-density 35 base pair probe spacing offers a more accurate view of protein/DNA interactions
- 25-mer probes yield the most specific hybridization results available
The GeneChip® Mouse Tiling 2.0R Array Set is designed for chromatin immunoprecipitation (ChIP) experiments. This set of seven arrays contains approximately 45 million oligonucleotide probes to interrogate the mouse genome. Each array in the set contains over 6.5 million perfect match probes to specifically interrogate genomic regions.
Sequences used in the design of the Mouse Tiling 2.0R Array Set were selected from NCBI mouse genome assembly (Build 33). Repetitive elements were removed by RepeatMasker. The Mouse Tiling 2.0R Array Set is available either as a whole-genome set of seven arrays (P/N 900852), or individual arrays from the set may be purchased separately.
The GeneChip® Mouse Promoter 1.0R Array is a subset of the Mouse Tiling 2.0R Tiling Array Set, and the content was chosen specifically to analyze protein/DNA interactions within promoter regions.
Probes are tiled at an average resolution of 35 base pairs, as measured from the central position of adjacent 25-mer oligos, leaving a gap of approximately 10bp between probes. Oligonucleotide probes are synthesized in situ complementary to each corresponding sequence.
Instrument and Software Requirements
Visit the Affymetrix ChIP-on-Chip Web Forum and share protocols, experiences, hints and tips on best practices for ChIP-on-chip and MeDIP-on-chip applications. Free registration is required to view or create posts.
Archived Presentations
Scientific Forum, 1st European ChIP-on-chip
Webinar, Six-part ChIP-on-chip Symposia Series
Application Notes
| Part # | Description | Details |
|---|---|---|
| 900852 | GeneChip® Mouse Tiling 2.0R Array Set | Complete A-G genome set - 7 Arrays |
| 900894 | GeneChip® Mouse Tiling 2.0R A Array | Covers chromosomes 1, 9, 19 - 6 Arrays |
| 900896 | GeneChip® Mouse Tiling 2.0R B Array | Covers chromosomes 2, Mitochondria, X, Y, and unassigned regions - 6 Arrays |
| 900897 | GeneChip® Mouse Tiling 2.0R C Array | Covers chromosomes 3, 7 18 - 6 Arrays |
| 900895 | GeneChip® Mouse Tiling 2.0R D Array | Covers chromosomes 4, 11, 17 - 6 Arrays |
| 900898 | GeneChip® Mouse Tiling 2.0R E Array | Covers chromosomes 5, 12, 15 - 6 Arrays |
| 900899 | GeneChip® Mouse Tiling 2.0R F Array | Covers chromosomes 6, 8, 16 - 6 Arrays |
| 900900 | GeneChip® Mouse Tiling 2.0R G Array | Covers chromosomes 10, 13, 14 - 6 Arrays |
Related Products
| Part # | Description | Details |
|---|---|---|
| Integrated Genome Browser (IGB) | Available Via Download | |
| Affymetrix® Tiling Analysis Software | Available Via Download | |
| 900301 | Control Oligo B2, 3nM | Sufficient for 30 samples |
| 900812 | GeneChip® WT Double-Stranded DNA Terminal Labeling Kit | Sufficient for 30 reactions |
Manuals
Chromatin Immunoprecipitation Assay Protocol (pdf, 679 KB)
Tutorial
Tiling Analysis Software Tutorial
Material Safety Data Sheets
WT Double-Stranded cDNA Synthesis Kit (zip, 166 KB)
Library Files
GeneChip Tiling Arrays - Data Analysis Library File Updates
Mouse Genome Tiling 2.0 Array Set NCBIv36 bed file
Mouse Genome Tiling 2.0 Array Set NCBIv36 BPMAP files
Mouse Genome Tiling 2.0 Array Set NCBIv36 probe beds
Mouse Tiling 2.0R Array Set (zip, 1 GB)
Package Inserts
Mouse Tiling 2.0R Array Set (pdf, 247 KB)




