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Human Genome U133A 2.0 Array

The GeneChip® Human Genome U133A 2.0 Array is a single array representing 14,500 well-characterized human genes that can be used to explore human biology and disease processes. New design and reduced feature size mean that you can use smaller sample volumes than the previous HG®U133A Array without compromising performance.

Provides coverage of well-substantiated genes in the transcribed human genome on a single array
Analyzes the expression level of 18,400 transcripts and variants, including 14,500 well-characterized human genes
Comprised of more than 22,000 probe sets and 500,000 distinct oligonucleotide features
Use the Power of the Probe Set and get multiple independent measurements for each transcript that deliver the greatest accuracy and reproducibility of any microarray platform
All probe sets represented on the GeneChip® Human Genome U133A Array are identically replicated on the GeneChip Human Genome U133A 2.0 Array

Array Profile

Sequences used in the design of the array were selected from GenBank®, dbEST, and RefSeq. The sequence clusters were created from the UniGene database (Build 133, April 20, 2001) and then were refined by analysis and comparison with a number of other publicly available databases including the Washington University EST trace repository and the University of California, Santa Cruz Golden-Path human genome database (April 2001 release).

Instrument and Software Requirements

GeneChip® Scanner 3000, enabled for High-Resolution Scanning*
GeneChip® Command Console® Software (AGCC) including the GeneChip® Scanner 3000 High-Resolution Scanning Patch

*GeneChip Scanner 3000 High-Resolution Update is standard on all instruments shipped starting in September 2003 with serial number series 502. Previous versions, serial number series 501, will require the 00-0110 GeneChip Scanner 3000 High-Resolution Update to be installed.

For more information, please review the data sheet (pdf, 169 KB).

Data Sheets

Human Genome U133 Arrays (pdf, 1.76 MB)

Technical Notes

Design and Performance of the GeneChip® Human Genome U133 Plus 2.0 and Human Genome U133A 2.0 Array (pdf, 207 KB)

GeneChip® Expression Platform: Comparison, Evolution, and Performance (pdf, 269 KB)

Application Notes

Microarray Applications in Infectious Disease (pdf, 252 KB)

Microarrays in Cancer Research (pdf, 423 KB)

Part #	Description	Details
900471	Human Genome U133A 2.0 Array	contains 2 arrays
900468	Human Genome U133A 2.0 Array	contains 6 arrays
900469	Human Genome U133A 2.0 Array	contains 30 arrays

Required Products

Part #	Description	Details
00-0210	GeneChip® Scanner 3000, enabled for High-Resolution Scanning	See product page for more information
	GeneChip® Command Console® Software (AGCC)	See product page for more information

Related Products

Part #	Description	Details
	Assays and Reagents

FAQs

Gene Expression Arrays

Human Genome U133 2.0 Arrays

Manuals

3' IVT Express Kit User Manual (pdf, 4.28 MB)

Alignment Data in PSL Format

Expression Analysis Technical Manual, with Specific Protocols for Use with the Hybridization, Wash, and Stain Kit (pdf, 1.70 MB)

HT 3' IVT Express Kit (pdf 33.6 MB)

Probe Set Data in MAGE-ML Format

Library Files

Human Genome U133A 2.0 Array (zip, 13 MB)

Package Inserts

Human Genome U133A 2.0 Array (pdf, 492 KB)

Alignment, Annotation, and Sequence Files

Information about using Alignment, Annotation, and Sequence files

NetAffx Alignment Files

HG-U133A_2 Alignments to Genome, PSL (3.7 MB, 9/28/06)

Current NetAffx Annotation Files

HG-U133A_2 Annotations, CSV format (11 MB, 3/12/09)

HG-U133A_2 Annotations, MAGE-ML XML Format (45 MB, 3/12/09)

HG-U133A_2 BLASTP annotations, CSV Format (2.9 KB, 3/4/09)

HG-U133A_2 Orthologs/Homologs, CSV Format (4.9 MB, 3/12/09)

Note: NetAffx annotation files are intended for use in automated analysis. Some are not compatible with all spreadsheet applications. Please use the NetAffx Analysis Center to limit download data to your probe sets of interest. Annotation CSV files for the exon arrays are split into a probe set-level annotation file and a transcript cluster-level annotation file; exon array CSV files are compatible with ExACT.