- High density 35-bp probe spacing offers a more accurate view of novel RNA transcripts or protein/DNA interaction
- 25-mer probes yield the most specific hybridization results.
The GeneChip® Chromosome 21/22 1.0 Array Set is a three array set designed for transcript mapping or chromatin immunoprecipitation (ChIP) experiments. This array set has been cited in many peer-reviewed publications; references are available using the Affymetrix publication database. Each package of six arrays contains two complete three array sets.
Sequences used in the design of the Chromosome 21/22 1.0 Array Set were selected from NCBI human genome assembly (Build 28). Repetitive elements were removed by RepeatMasker. This set of three arrays contains over one million probe pairs to interrogate chromosomes 21 and 22 at approximately 35-base pair resolution. Probes are tiled at 35-bp resolution, as measured from the central position of adjacent 25-mer oligos, creating a gap of approximately 10 bp between probes.
Two versions of the set are available; Chromosome 21/22 1.0F Array Set (P/N 900545) is complementary to the forward (+) strand of the human genome while Chromosome 21/22 1.0R Array Set (P/N 900546) is complementary to the reverse (-) strand.
Instrument and Software Requirements
- GeneChip® Scanner 3000 enabled for High-Resolution Scanning*
- GeneChip® Command Console® Software (AGCC)
*GeneChip Scanner 3000 High-Resolution Update is standard on all instruments shipped starting in September 2003 with serial number series 502. Previous versions, serial number series 501, will require the 00-0110 GeneChip Scanner 3000 High-Resolution Update to be installed.
|900546||GeneChip® Chromosome 21/22 1.0R Array Set||6 arrays|
|900545||GeneChip® Chromosome 21/22 1.0F Array Set||6 arrays|
|900301||Control Oligo B2, 3nM||Sufficient for 30 samples|
|900811||GeneChip® WT Amplified Double-Stranded cDNA Synthesis Kit||Sufficient for 10 reactions|
|900812||GeneChip® WT Double-Stranded DNA Terminal Labeling Kit||Sufficient for 30 reactions|
|900813||GeneChip® WT Double-Stranded cDNA Synthesis Kit||Sufficient for 30 reactions|
|Affymetrix® Tiling Array Tools||Available Via Download|
|Integrated Genome Browser (IGB)||Available Via Download|